| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vetkoetter, Clare M (17)
|
NTTFA |
53.30 |
55.88 |
2.58 |
| 2 |
Hood, Jenna C (17)
|
NTTFA |
52.87 |
56.03 |
3.16 |
| 3 |
Oushalkas, Maria Jhoanne V (15)
|
NTTFA |
54.45 |
57.24 |
2.79 |
| 4 |
Simatacolos, Eleni A (15)
|
NTTFA |
56.28 |
57.67 |
1.39 |
| 5 |
Giachini, Sabrina R (16)
|
NTTFA |
56.34 |
57.72 |
1.38 |
| 6 |
Tran, Kylie T (15)
|
NTTFA |
57.30 |
58.06 |
0.76 |
| 7 |
Morris, Logan A (15)
|
NTTFA |
56.68 |
58.27 |
1.59 |
| 8 |
Henderson, Filippa E (17)
|
NTTFA |
56.09 |
58.58 |
2.49 |
| 9 |
Blum, Victoria A (17)
|
NTTFA |
55.19 |
59.58 |
4.39 |
| 10 |
Kim, Ariana B (15)
|
NTTFA |
57.78 |
1:00.43 |
2.65 |
| 11 |
Ballantyne, Emma A (17)
|
NTTFA |
56.63 |
1:00.50 |
3.87 |
| 12 |
Michaels, Lauren (18)
|
NTTFA |
55.67 |
1:00.51 |
4.84 |
| 13 |
Draper, Rachel P (17)
|
NTTFA |
1:00.15 |
1:00.95 |
0.80 |
| 13 |
Kipe, Channing O (17)
|
NTTFA |
58.39 |
1:00.95 |
2.56 |
| 15 |
Hunt, Theresa R (17)
|
NTTFA |
58.34 |
1:01.09 |
2.75 |
| 16 |
Mok, Erin (16)
|
NTTFA |
58.11 |
1:01.10 |
2.99 |
| 17 |
Sitz, Bethany M (15)
|
NTTFA |
59.40 |
1:01.81 |
2.41 |
| 18 |
Scherman, Devin L (17)
|
NTTFA |
57.06 |
1:01.93 |
4.87 |
| 19 |
Reizer, Eliana R (15)
|
NTTFA |
58.71 |
1:02.35 |
3.64 |
| 20 |
Warren, Camryn Z (15)
|
NTTFA |
1:04.53 |
1:05.27 |
0.74 |