| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Richner, Lauren E (17)
|
OHWOR |
33.00 |
31.88 |
-1.12 |
| 2 |
Lichtner, Kasey E (18)
|
OHGCST |
36.35 |
32.39 |
-3.96 |
| 3 |
Bosler, Madelyn R (15)
|
OHWAC |
34.77 |
34.13 |
-0.64 |
| 4 |
Schirtzinger, Eloise R (15)
|
OHWAC |
41.05 |
34.19 |
-6.86 |
| 5 |
Butnariu, Alexandra (17)
|
OHGCST |
34.51 |
35.02 |
0.51 |
| 6 |
York, Samantha P (15)
|
OHGCST |
39.24 |
35.77 |
-3.47 |
| 7 |
Gabriel, Holly L (17)
|
OHWOR |
34.23 |
36.62 |
2.39 |
| 8 |
Elliott, Olivia K (15)
|
OHWOR |
37.26 |
38.77 |
1.51 |
| 9 |
Neese, Chayse J (16)
|
OHGCST |
43.12 |
39.35 |
-3.77 |
| 10 |
Marquardt, Abigail G (15)
|
OHGCST |
|
41.74 |
- |
|
Browning, Kate A (16)
|
OHWOR |
43.20 |
NS |
- |
|
Ebersole, Lauryn M (15)
|
OHWOR |
35.00 |
NS |
- |