| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blackford, Peiffer A (11)
|
OHWAC |
2:56.40 |
2:49.76 |
-6.64 |
| 2 |
Espy, Adelyn G (11)
|
OHWAC |
2:58.16 |
2:53.03 |
-5.13 |
| 3 |
Davidson, Madeline N (11)
|
OHWAC |
3:00.21 |
2:57.44 |
-2.77 |
| 4 |
Penrod, Anna R (12)
|
OHUASC |
3:45.00 |
2:58.59 |
-46.41 |
| 5 |
Sullivan, Sophia I (11)
|
OHBKYS |
2:54.54 |
3:00.17 |
5.63 |
| 6 |
Claggett, Brookelyn O (12)
|
OHBKYS |
2:53.67 |
3:02.13 |
8.46 |
| 7 |
Kelly, Hannah E (11)
|
OHWAC |
3:05.02 |
3:05.56 |
0.54 |
| 8 |
Andersson, Elisabeth R (12)
|
OHUASC |
3:00.00 |
3:08.04 |
8.04 |
| 9 |
Benz, Elena R (12)
|
OHBKYS |
3:25.30 |
3:18.25 |
-7.05 |
| 10 |
McDonald, Lily P (11)
|
OHBKYS |
3:00.43 |
3:18.40 |
17.97 |
| 11 |
Jack, Audrey Y (12)
|
OHUASC |
4:00.00 |
3:28.82 |
-31.18 |
| 12 |
Patel, Kaylah L (11)
|
OHGCST |
|
3:34.12 |
- |
| 13 |
Bernard, Emily O (12)
|
OHBKYS |
3:40.00 |
3:36.47 |
-3.53 |
| 14 |
Haverick, Naomi R (11)
|
OHUASC |
3:45.00 |
3:44.99 |
-0.01 |
| 15 |
Coverdale, Anna Caroline E (12)
|
OHWAC |
3:28.21 |
3:52.37 |
24.16 |
|
Bollinger, Brynn M (12)
|
OHBKYS |
3:01.10 |
DQ |
- |