| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mumaw, Ashton M (12)
|
OHUASC |
6:00.00 |
5:27.87 |
-32.13 |
| 2 |
Blackford, Peiffer A (11)
|
OHWAC |
5:39.15 |
5:32.19 |
-6.96 |
| 3 |
Rajaobelina, Jessica A (11)
|
OHWAC |
5:48.21 |
5:36.84 |
-11.37 |
| 4 |
Foss, Natalie M (11)
|
OHUASC |
6:15.00 |
5:43.90 |
-31.10 |
| 5 |
Claggett, Brookelyn O (12)
|
OHBKYS |
6:05.05 |
5:47.71 |
-17.34 |
| 6 |
Davidson, Madeline N (11)
|
OHWAC |
5:57.21 |
5:47.88 |
-9.33 |
| 7 |
Benz, Elena R (12)
|
OHBKYS |
5:50.57 |
5:48.77 |
-1.80 |
| 8 |
Seelandt, Ella M (11)
|
OHBKYS |
6:01.82 |
5:50.07 |
-11.75 |
| 9 |
Gerlach, Eloise G (12)
|
OHWOR |
6:15.00 |
5:56.87 |
-18.13 |
| 10 |
Fleming, Mia E (12)
|
OHUASC |
6:15.00 |
5:57.53 |
-17.47 |
| 11 |
Cirman, Ellie M (12)
|
OHBKYS |
5:56.82 |
5:59.05 |
2.23 |
| 12 |
Thomas, Kyli M (11)
|
OHWAC |
7:03.78 |
6:01.40 |
-62.38 |
| 13 |
McDonald, Lily P (11)
|
OHBKYS |
6:04.32 |
6:02.84 |
-1.48 |
| 14 |
Hammer, Layla F (11)
|
OHWAC |
6:23.30 |
6:06.87 |
-16.43 |
| 15 |
Sullivan, Sophia I (11)
|
OHBKYS |
6:09.02 |
6:07.94 |
-1.08 |
| 16 |
Bollinger, Brynn M (12)
|
OHBKYS |
6:10.00 |
6:12.72 |
2.72 |
| 17 |
Browning, Anna E (12)
|
OHWOR |
6:15.00 |
6:18.16 |
3.16 |
| 18 |
Reynolds, Claire S (11)
|
OHBKYS |
6:41.45 |
6:37.50 |
-3.95 |
| 19 |
Wolfe, Emily E (12)
|
OHLTPY |
7:54.00 |
7:17.39 |
-36.61 |