| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yurkovich, Hailey L (14)
|
AMGYST |
4:54.98 |
4:39.70 |
-15.28 |
| 2 |
Crosscut, Kaitlyn M (13)
|
COUN |
5:06.16 |
4:48.74 |
-17.42 |
| 3 |
Bowser, Lauren (14)
|
RGS |
4:57.23 |
4:52.06 |
-5.17 |
| 4 |
Marzolf, Lilah A (14)
|
AMBYAC |
5:12.02 |
5:10.66 |
-1.36 |
| 5 |
McCombs, Riley (13)
|
NCCY |
5:16.30 |
5:12.62 |
-3.68 |
| 6 |
Watts, Abigail (14)
|
AVY |
5:15.74 |
5:12.93 |
-2.81 |
| 7 |
Stewart, Ashley J (14)
|
AMRESY |
5:32.44 |
5:16.10 |
-16.34 |
| 8 |
Russell, Emily (13)
|
OCY |
5:40.83 |
5:17.77 |
-23.06 |
| 9 |
Frazer, Grace M (13)
|
AMINDY |
5:32.10 |
5:19.42 |
-12.68 |
| 10 |
Svolos, Sydney (14)
|
OCY |
5:35.85 |
5:20.62 |
-15.23 |
| 11 |
Tinstman, Bella (14)
|
NCCY |
5:31.98 |
5:23.57 |
-8.41 |
| 12 |
Golier, Madeline S (14)
|
BRK |
5:37.28 |
5:27.25 |
-10.03 |
| 13 |
Vaniddekinge, McKenzie J (14)
|
AMRESY |
5:38.18 |
5:31.71 |
-6.47 |
| 14 |
Polanofsky, Krista E (14)
|
AMGYST |
5:43.59 |
5:35.17 |
-8.42 |
| 15 |
Birch, Autumn (14)
|
AMBYAC |
5:40.67 |
5:35.26 |
-5.41 |
|
Bailey, Peytyn C (13)
|
MYST |
5:28.42 |
DQ |
- |
|
DiMaio, Eva (13)
|
RGS |
5:32.22 |
DQ |
- |