| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sens, Natalie R (12)
|
AMPEAQ |
2:38.33 |
2:34.74 |
-3.59 |
| 2 |
Li, Lilian Y (11)
|
LELESD |
2:41.97 |
2:35.98 |
-5.99 |
| 3 |
Marcos, Isabella S (12)
|
AMKW |
2:45.73 |
2:37.39 |
-8.34 |
| 4 |
Chen, Grace Y (12)
|
LESTRS |
2:44.46 |
2:39.27 |
-5.19 |
| 5 |
Stewart, Beatrice P (12)
|
LEGLSS |
2:46.34 |
2:44.49 |
-1.85 |
| 6 |
King, Abigail (12)
|
AMCSC |
2:49.77 |
2:48.61 |
-1.16 |
| 7 |
Kunkle, Paige K (11)
|
AMFAST |
2:55.30 |
2:49.04 |
-6.26 |
| 8 |
Gilligan, Rena A (12)
|
AMUN-M |
2:57.21 |
2:49.22 |
-7.99 |
| 9 |
Lowe, Kate E (12)
|
AMFAST |
2:58.49 |
2:50.58 |
-7.91 |
| 10 |
Bunker, Kate L (11)
|
AMFAST |
2:52.93 |
2:52.64 |
-0.29 |
| 11 |
Sammut, Madeline (12)
|
LEGO |
2:59.06 |
2:52.90 |
-6.16 |
| 12 |
Pazuchanics, Kathryn E (12)
|
AMPTSC |
2:52.88 |
2:53.14 |
0.26 |
| 13 |
Smith, Audrie E (12)
|
WVFAST |
3:22.13 |
2:53.97 |
-28.16 |
| 14 |
Gracey-Dalton, Karsyn A (11)
|
NIBRAD |
3:32.35 |
2:55.54 |
-36.81 |
| 15 |
Pesek, zoe A (12)
|
LEUSC |
2:53.29 |
2:56.01 |
2.72 |
| 16 |
Cox, Caitlin L (11)
|
LECCS |
3:06.20 |
2:56.21 |
-9.99 |
| 17 |
Jordan, Jaidyn L (12)
|
LEUN |
2:54.51 |
2:56.56 |
2.05 |
| 18 |
Polyakov, Sophia S (12)
|
LESTRS |
3:03.38 |
2:56.91 |
-6.47 |
| 19 |
Gustin, Jana R (12)
|
LEGLSS |
3:08.24 |
2:57.46 |
-10.78 |
| 20 |
Metzler, Maya K (12)
|
LEHEAT |
3:01.76 |
2:57.78 |
-3.98 |
| 21 |
Sipple, Lauren M (12)
|
AMGPAC |
3:06.52 |
2:58.24 |
-8.28 |
| 22 |
Moffat, Madison O (12)
|
AMBPR |
3:03.08 |
3:02.72 |
-0.36 |
| 23 |
Price, Erin C (12)
|
AMFAST |
3:07.46 |
3:02.94 |
-4.52 |
| 24 |
Houser, McKenna L (12)
|
AMMOON |
|
3:03.35 |
- |
| 25 |
Sieger, Annabelle E (11)
|
LELESD |
3:08.49 |
3:03.82 |
-4.67 |
| 26 |
Moldenhauer, Paige A (12)
|
NIEAST |
3:03.87 |
3:05.37 |
1.50 |