| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scott, Peyton R (13)
|
INDY |
1:09.00 |
1:09.88 |
0.88 |
| 2 |
Lavorini, Giona (13)
|
AMBYAC |
1:10.10 |
1:10.43 |
0.33 |
| 3 |
Burns, Ann-Katherine (14)
|
AMBYAC |
1:15.78 |
1:10.98 |
-4.80 |
| 4 |
Walko, Haley L (13)
|
AMGYST |
1:18.73 |
1:14.86 |
-3.87 |
| 5 |
Long, Hadley (14)
|
INDY |
1:15.68 |
1:15.47 |
-0.21 |
| 6 |
Shipe, Bryony S (13)
|
AMRGSY |
1:17.27 |
1:15.99 |
-1.28 |
| 7 |
Fraundorfer, Alexis R (14)
|
AMBRY |
1:17.17 |
1:16.06 |
-1.11 |
| 8 |
Alfera, Sydney (14)
|
LWCY |
1:17.92 |
1:17.88 |
-0.04 |
| 9 |
Speer, Ellen (13)
|
INDY |
1:17.52 |
1:18.57 |
1.05 |
| 10 |
Altman, Mary Anne (13)
|
MVY |
1:19.35 |
1:19.37 |
0.02 |
| 11 |
Kohan, Karlee E (14)
|
AMGJY |
1:17.24 |
1:19.80 |
2.56 |
| 12 |
Symchak, Victoria H (13)
|
AMRESY |
1:21.27 |
1:19.99 |
-1.28 |
| 13 |
Parson, Kaitlyn (14)
|
AMBYAC |
1:18.77 |
1:20.43 |
1.66 |
| 14 |
Birch, Rachel (14)
|
AMBYAC |
1:21.48 |
1:20.73 |
-0.75 |