| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McBeth, Conner (18)
|
LWCY |
20.36 |
20.66 |
0.30 |
| 2 |
Thome, Kyle (17)
|
INDY |
21.72 |
21.23 |
-0.49 |
| 3 |
Griffith, Isaac (17)
|
INDY |
22.76 |
22.26 |
-0.50 |
| 4 |
LaPietra, Julian (16)
|
LWCY |
22.85 |
22.54 |
-0.31 |
| 5 |
Hamilton, Ian E (15)
|
AMUNTY |
23.45 |
22.80 |
-0.65 |
| 5 |
Innis, Elijah J (16)
|
AMGJY |
23.37 |
22.80 |
-0.57 |
| 7 |
Zilch, Herman IV G (17)
|
AMGJY |
23.70 |
23.08 |
-0.62 |
| 7 |
Atkinson, Preston M (17)
|
AMGYST |
22.70 |
23.08 |
0.38 |
| 9 |
Littleton, Matthew J (16)
|
AMRESY |
22.70 |
23.17 |
0.47 |
| 10 |
Frederick, Aaron P (17)
|
AMSPY |
23.59 |
23.25 |
-0.34 |
| 11 |
Silsby, Nathan O (16)
|
AMRESY |
23.76 |
23.91 |
0.15 |
| 12 |
Locke, Connor (15)
|
AMUNTY |
24.64 |
24.13 |
-0.51 |
| 13 |
Rogers, Jordan M (17)
|
AMSPY |
23.39 |
24.15 |
0.76 |
| 14 |
Smith, Evan R (17)
|
AMSPY |
24.19 |
24.18 |
-0.01 |
| 15 |
Weigel, Parker A (16)
|
AMRGSY |
24.54 |
24.30 |
-0.24 |
| 16 |
Schiffbauer, Jacob M (15)
|
AMUNTY |
24.24 |
24.34 |
0.10 |
| 17 |
Grimes, Dalton (16)
|
AMUNTY |
24.31 |
24.40 |
0.09 |
| 18 |
O'Stafy, Nathaniel T (17)
|
AMGJY |
24.41 |
24.42 |
0.01 |
| 19 |
Webb, Ben R (15)
|
AMRGSY |
24.25 |
24.54 |
0.29 |
| 20 |
Tucker, Ryan M (16)
|
AMGYST |
24.41 |
24.69 |
0.28 |
| 21 |
Guise, Brandon X (16)
|
AMRESY |
24.85 |
25.06 |
0.21 |
| 22 |
Snively, Logan (15)
|
AMGYST |
24.87 |
25.27 |
0.40 |
| 23 |
Clay, Kairon (18)
|
ZZTLYZ |
24.43 |
26.01 |
1.58 |