| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hewitt, Briella J (11)
|
LEGO |
27.02 |
26.71 |
-0.31 |
| 2 |
Swartzwelder, Laina C (12)
|
LEGYB |
29.10 |
28.84 |
-0.26 |
| 3 |
Cerone, Abby G (11)
|
LEGYB |
30.55 |
29.24 |
-1.31 |
| 4 |
Parkinson, Lilly (12)
|
OHLCCY |
31.34 |
30.04 |
-1.30 |
| 5 |
Otradovec, Hailey (11)
|
WAD |
32.69 |
30.77 |
-1.92 |
| 6 |
Cassidy, Anna (11)
|
OHLCCY |
31.40 |
31.00 |
-0.40 |
| 7 |
Stupi, Abigail C (12)
|
LEGO |
32.22 |
31.95 |
-0.27 |
| 8 |
Francis, Kaitlynn (12)
|
OHLCCY |
33.43 |
32.04 |
-1.39 |
| 9 |
Sines, Abby (11)
|
WAD |
33.63 |
32.65 |
-0.98 |
| 10 |
Belden, Paige (12)
|
OHMLY |
32.92 |
32.79 |
-0.13 |
| 11 |
Ketler, Natalie M (11)
|
LEGYB |
33.19 |
33.49 |
0.30 |
| 12 |
Cole, Sydney (11)
|
LEGYB |
33.13 |
33.74 |
0.61 |
| 13 |
Siebert, Julia M (11)
|
LEGO |
36.31 |
34.55 |
-1.76 |
| 14 |
Madrigal, Stellaluna (11)
|
OHAY |
34.88 |
34.79 |
-0.09 |
| 15 |
Sliwinski, Samantha (11)
|
OHMLY |
36.28 |
37.02 |
0.74 |
| 16 |
McElroy, Savanna (12)
|
LCE |
40.12 |
37.72 |
-2.40 |
| 17 |
Ruzin, Anne (11)
|
LCE |
40.09 |
39.36 |
-0.73 |
| 18 |
Collins, Abigail (11)
|
WAD |
40.01 |
40.24 |
0.23 |
| 19 |
Daw, Molly (11)
|
WAD |
38.77 |
40.30 |
1.53 |
| 20 |
Thomas, Mia S (11)
|
LEGO |
40.51 |
40.76 |
0.25 |
| 21 |
Elek, Norah (11)
|
OHMLY |
45.90 |
40.87 |
-5.03 |
| 22 |
Allison, Jade (11)
|
OHMLY |
39.07 |
41.55 |
2.48 |
| 23 |
Iskra, Evie M (11)
|
LEGO |
42.44 |
42.40 |
-0.04 |
| 24 |
Gasser, Rebecca (11)
|
WAD |
49.95 |
42.64 |
-7.31 |
| 25 |
Sedivy, Nola (11)
|
LCE |
47.20 |
43.61 |
-3.59 |
| 26 |
Studd, Kylee (12)
|
WAD |
41.58 |
43.84 |
2.26 |
| 27 |
Preston, Emma G (11)
|
LEGO |
42.57 |
44.49 |
1.92 |
| 28 |
Raymond, Angel D (12)
|
LEGO |
41.62 |
46.53 |
4.91 |
|
Kaiser, Kendall (12)
|
OHLCCY |
43.26 |
NS |
- |
|
Riedl, Abigail A (11)
|
LEGYB |
42.03 |
NS |
- |