| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eckenrode, Jordan K (10)
|
AMSRST |
39.70 |
38.67 |
-1.03 |
| 2 |
Hagenstein, Carson B (10)
|
AMWAAC |
43.74 |
43.62 |
-0.12 |
| 3 |
McFate, Brady R (10)
|
AMWAAC |
48.21 |
46.51 |
-1.70 |
| 4 |
Brooks, Gannon L (10)
|
AMPRA |
58.11 |
46.96 |
-11.15 |
| 5 |
Haspel, Logan M (10)
|
AMWAAC |
47.25 |
47.70 |
0.45 |
| 6 |
Khalid, Taimur A (10)
|
AMPRA |
55.56 |
49.16 |
-6.40 |
| 7 |
Robertson, Derek J (10)
|
AMWAAC |
1:06.14 |
51.43 |
-14.71 |
| 8 |
Browning-Dias, Brandon K (10)
|
AMJCCS |
1:00.60 |
52.29 |
-8.31 |
| 9 |
Gross, Vincent R (10)
|
AMPTSC |
1:02.00 |
53.99 |
-8.01 |
| 10 |
Lentz, Carson A (10)
|
AMPRA |
1:00.48 |
55.63 |
-4.85 |
| 11 |
Hetrick, William R (10)
|
AMPRA |
1:02.08 |
58.04 |
-4.04 |
| 12 |
Butts, Joseph T (10)
|
AMWAAC |
1:15.32 |
59.31 |
-16.01 |
| 13 |
Vacula, Bryce M (10)
|
AMPRA |
57.19 |
1:01.62 |
4.43 |
|
Webster, Holden G (10)
|
AMHAAC |
45.86 |
DQ |
- |
|
Farrar, Lachlan A (10)
|
AMPRA |
55.19 |
NS |
- |