| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schomaker, Allison H (13)
|
AMPRA |
32.04 |
31.33 |
-0.71 |
| 2 |
Driscoll, Emma L (13)
|
AMHAAC |
|
32.89 |
- |
| 3 |
Shafer, Riley A (13)
|
AMPRA |
34.19 |
33.32 |
-0.87 |
| 4 |
Magistro, Sophia R (13)
|
AMHAAC |
34.95 |
33.36 |
-1.59 |
| 5 |
Heller-Matza, Lilja S (13)
|
AMJCCS |
38.22 |
34.08 |
-4.14 |
| 6 |
Rupert, Brooke T (13)
|
AMPRA |
39.14 |
34.49 |
-4.65 |
| 7 |
Quemado, Dalisay B (13)
|
AMPRA |
40.84 |
37.20 |
-3.64 |
| 8 |
Eichelberger, Isabella K (13)
|
AMNAC |
40.29 |
40.07 |
-0.22 |
| 9 |
Alvarez, Sara L (13)
|
AMPRA |
43.03 |
40.80 |
-2.23 |
| 10 |
Rapneth, Kylie R (13)
|
AMCSC |
41.23 |
44.35 |
3.12 |
| 11 |
Konggaard, Annemarie (13)
|
AMPTSC |
57.09 |
47.40 |
-9.69 |
| 12 |
Khalid, Layla A (13)
|
AMPRA |
51.10 |
48.30 |
-2.80 |
|
Fleeger, Miranda A (13)
|
AMSRST |
41.07 |
NS |
- |