| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bissett, Ainsley L (16)
|
AMPRA |
5:24.17 |
5:24.61 |
0.44 |
| 2 |
Della Toffalo, Cara J (15)
|
AMRESY |
5:28.74 |
5:42.78 |
14.04 |
| 3 |
Toth, Ashlee A (16)
|
AMEFSC |
5:39.17 |
5:46.13 |
6.96 |
| 4 |
Nebiolo, Lydia D (16)
|
AMSASC |
5:30.34 |
5:46.56 |
16.22 |
| 5 |
Moretti, Madison E (17)
|
AMCSC |
5:49.86 |
5:47.98 |
-1.88 |
| 6 |
Kohler, Anna C (15)
|
AMPEAQ |
6:00.85 |
5:58.66 |
-2.19 |
| 7 |
Connelly, Riley A (15)
|
AMPRA |
5:47.94 |
5:59.00 |
11.06 |
| 8 |
Baiano, Gabriella L (16)
|
AMSFAC |
5:44.59 |
6:01.29 |
16.70 |
| 9 |
Zbezinski, Valerie M (17)
|
AMPRA |
5:51.45 |
6:01.62 |
10.17 |
| 10 |
Jackovic, Emily A (16)
|
AMSPAC |
6:16.16 |
6:01.87 |
-14.29 |
| 11 |
Majors, Allison E (15)
|
AMRESY |
6:19.00 |
6:09.31 |
-9.69 |
| 12 |
Burk, Abby N (16)
|
AMUN-R |
5:54.06 |
6:20.65 |
26.59 |
| 13 |
Caldwell, Kelsey J (15)
|
AMBPR |
6:30.14 |
6:23.32 |
-6.82 |
| 14 |
Love, Madison E (16)
|
AMBPR |
6:02.45 |
6:24.75 |
22.30 |