| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Debaecke, Sydney E (10)
|
MADST |
5:44.11 |
5:40.41 |
-3.70 |
| 2 |
Waslyn, Rylee E (10)
|
MAWAC |
5:46.95 |
5:43.88 |
-3.07 |
| 3 |
Brandt, Mackenzie A (10)
|
MAWAC |
6:11.69 |
5:56.63 |
-15.06 |
| 4 |
Derivaux, Lillian E (10)
|
MAJW |
6:10.05 |
5:59.65 |
-10.40 |
| 5 |
Ryan, Lenya H (10)
|
MADST |
5:57.76 |
6:00.26 |
2.50 |
| 6 |
Phelps, Anna E (9)
|
MAJW |
6:24.25 |
6:05.62 |
-18.63 |
| 7 |
Marlin, Krista N (10)
|
MAGRA |
6:21.68 |
6:07.01 |
-14.67 |
| 8 |
Staretz, Presley E (10)
|
MAEPAC |
6:23.84 |
6:13.70 |
-10.14 |
| 9 |
Shoemaker, Jennifer E (10)
|
MAWSY |
6:07.94 |
6:15.29 |
7.35 |
| 10 |
Wilson, Mekhi S (10)
|
MASSC |
6:20.65 |
6:18.05 |
-2.60 |
| 11 |
Reteneller, Riley T (10)
|
MACBST |
6:32.38 |
6:23.61 |
-8.77 |
| 12 |
Agne, Kira W (10)
|
MAWAC |
6:25.18 |
6:27.10 |
1.92 |
| 13 |
Olhasso, Miranda P (10)
|
MAGSA |
6:47.81 |
6:29.87 |
-17.94 |
| 14 |
Mulhern, Jenna (10)
|
MAWAP |
6:32.34 |
6:29.88 |
-2.46 |
| 15 |
Waggoner, Madeline M (10)
|
MAJW |
6:31.29 |
6:31.08 |
-0.21 |
| 16 |
Stressman, Kayla M (9)
|
MANPAC |
6:30.47 |
6:35.10 |
4.63 |
| 17 |
Shaffer, Marissa K (10)
|
MAPCAC |
6:37.68 |
6:38.63 |
0.95 |
| 18 |
Testa, Madison N (10)
|
MADST |
6:41.07 |
6:39.98 |
-1.09 |
| 19 |
Brandbergh, Analiese P (10)
|
MATHY |
6:43.72 |
6:41.14 |
-2.58 |
| 20 |
Kalish, Lindsay P (10)
|
MADST |
6:45.93 |
6:41.46 |
-4.47 |
| 21 |
Feinberg, Lindsay T (10)
|
MAEAAC |
6:40.17 |
6:41.79 |
1.62 |
| 22 |
Dipietro, Sydney M (10)
|
MAWAC |
6:47.24 |
6:45.65 |
-1.59 |
| 23 |
Surfus, Jordyn M (10)
|
MAHAC |
6:44.23 |
6:47.06 |
2.83 |
| 24 |
Naveen, Annika (10)
|
MAFCA |
6:40.55 |
6:55.56 |
15.01 |
| 25 |
Ober, Jenna E (10)
|
MAFSSC |
6:47.79 |
7:03.96 |
16.17 |