| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sullivan, Nolan M (9)
|
NIHAMB |
23.68 |
22.61 |
-1.07 |
| 2 |
Vitko, Kaden L (10)
|
NIHAMB |
29.76 |
26.23 |
-3.53 |
| 3 |
Jacobs, Carson C (6)
|
NITTAC |
33.03 |
26.63 |
-6.40 |
| 4 |
Neuhaus, Claire K (8)
|
NIGISC |
24.84 |
27.81 |
2.97 |
| 5 |
Vandriel, Annabelle k (8)
|
NIGISC |
26.30 |
28.16 |
1.86 |
| 6 |
Stockinger, Audrey M (9)
|
NIGISC |
26.88 |
29.32 |
2.44 |
| 7 |
Cook, Hannah R (7)
|
NIHAMB |
26.62 |
29.50 |
2.88 |
| 8 |
Zent, Madison L (8)
|
NIHAMB |
33.69 |
30.05 |
-3.64 |
| 9 |
Bronson, Maxwell F (10)
|
NIGISC |
31.13 |
30.58 |
-0.55 |
| 10 |
Kleinmann, Gabrielle C (6)
|
NIHAMB |
|
45.17 |
- |
| 11 |
Antonucci, Tyler J (6)
|
NIHAMB |
31.90 |
45.41 |
13.51 |
|
Rusert, Emerson K (8)
|
NIGISC |
|
DQ |
- |
|
Vitko, Brodie F (8)
|
NIHAMB |
28.00 |
DQ |
- |
|
Heider, Andrew J (10)
|
NIHAMB |
|
NS |
- |
|
Heider, Nathan E (9)
|
NIHAMB |
26.01 |
NS |
- |
|
Kleinmann, Samantha E (7)
|
NIHAMB |
|
NS |
- |