| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rountree, Rori F (11)
|
NCUN |
40.02 |
40.00 |
-0.02 |
| 2 |
Misener, Katrin J (12)
|
NCLOY |
45.30 |
42.15 |
-3.15 |
| 3 |
Tucker, Meredith E (12)
|
NCLOY |
46.52 |
43.82 |
-2.70 |
| 4 |
Tidwell, Katherine E (12)
|
NCLOY |
|
43.85 |
- |
| 5 |
Williams, Jordan G (11)
|
NCGCY |
|
44.89 |
- |
| 6 |
Shaw, Patricia G (11)
|
NCGCY |
|
46.04 |
- |
| 7 |
Respess, Josephine G (12)
|
NCLOY |
48.23 |
46.21 |
-2.02 |
| 8 |
Bankirer, Ellery W (12)
|
NCLOY |
52.49 |
47.88 |
-4.61 |
| 9 |
Brewer, Kamryn B (11)
|
NCLOY |
49.77 |
48.17 |
-1.60 |
| 10 |
Cranford, Aurora G (12)
|
NCGCY |
49.87 |
48.65 |
-1.22 |
| 11 |
Russell, Leilani M (12)
|
NCLOY |
47.36 |
48.78 |
1.42 |
| 12 |
McDonald, Catherine E (11)
|
NCGCY |
|
49.06 |
- |
| 13 |
Whetstone, Hunter A (12)
|
NCLOY |
|
49.61 |
- |
| 14 |
Yeh, Sabrina T (11)
|
NCLOY |
|
51.45 |
- |
| 15 |
Mann, Kenzie B (12)
|
NCGCY |
50.93 |
51.70 |
0.77 |
| 16 |
Abernathy, Kate N (12)
|
NCGCY |
|
52.37 |
- |
| 17 |
Bush, Savanna N (11)
|
NCLOY |
|
52.49 |
- |
| 18 |
Brannen, Molly A (12)
|
NCLOY |
54.50 |
53.28 |
-1.22 |
| 19 |
Schuster, Ava P (12)
|
NCLOY |
52.67 |
54.85 |
2.18 |
| 20 |
Ballard, Lila N (12)
|
NCGCY |
54.31 |
55.00 |
0.69 |
| 21 |
Malloy, Adrienne S (11)
|
NCLOY |
|
55.73 |
- |
| 22 |
Wehe, Jadie R (11)
|
NCGCY |
|
55.74 |
- |
| 23 |
Gayford, Ellen B (11)
|
NCLOY |
|
56.37 |
- |
| 24 |
Hill, Kipley L (11)
|
NCGCY |
1:02.79 |
57.01 |
-5.78 |
| 25 |
Roley, Abigail S (11)
|
NCGCY |
1:09.27 |
1:03.70 |
-5.57 |