| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morrow, Connor E (12)
|
NCUN-0 |
28.46 |
26.47 |
-1.99 |
| 2 |
Kosmala, Henryk W (12)
|
NCWST |
27.04 |
27.80 |
0.76 |
| 3 |
Ramos, John M (12)
|
NCSFSC |
29.13 |
27.99 |
-1.14 |
| 4 |
Page, Benjamin L (11)
|
NCWBST |
31.17 |
28.26 |
-2.91 |
| 5 |
Ramos, Noah R (12)
|
NCSFSC |
30.50 |
29.61 |
-0.89 |
| 6 |
True, Nathaniel E (11)
|
NCTRY |
31.19 |
30.26 |
-0.93 |
| 7 |
Williams, Matheson E (11)
|
NCSTAR |
30.63 |
30.36 |
-0.27 |
| 8 |
Auman, Isaiah H (12)
|
NCUN-0 |
31.71 |
30.60 |
-1.11 |
| 9 |
Williams, Brett T (12)
|
NCEAC |
32.53 |
31.33 |
-1.20 |
| 10 |
Rolland, Brady M (11)
|
NCSTAR |
32.15 |
32.30 |
0.15 |
| 11 |
Reynolds, Jonathan J (12)
|
NCSFSC |
32.82 |
32.60 |
-0.22 |
| 12 |
Croker, Ben M (12)
|
NCSFSC |
35.00 |
32.93 |
-2.07 |
| 13 |
Bruton, Kane S (11)
|
NCSTAR |
|
34.40 |
- |
| 14 |
Fernando, Benjamin E (12)
|
NCSFSC |
34.77 |
34.87 |
0.10 |
| 15 |
Lawson, Press J (11)
|
NCSTAR |
|
36.02 |
- |
| 16 |
Land, Matthew C (11)
|
NCSTAR |
37.23 |
37.46 |
0.23 |
| 17 |
Newton, Lucas A (11)
|
NCSTAR |
36.98 |
37.73 |
0.75 |
| 18 |
Taylor, Kadence M (11)
|
NCSTAR |
37.95 |
37.97 |
0.02 |
| 19 |
Ellis, Andrew T (11)
|
NCYBAC |
46.74 |
41.10 |
-5.64 |
| 20 |
Fulton, William F (11)
|
NCSFSC |
|
1:18.06 |
- |