| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Snyder, Rachel A (16)
|
NCLOY |
25.55 |
26.54 |
0.99 |
| 2 |
Rasbornik, Kensley K (17)
|
NCLOY |
25.02 |
28.43 |
3.41 |
| 3 |
Claudy, Madison A (15)
|
NCUN |
27.93 |
28.60 |
0.67 |
| 4 |
Fortin, Nicole E (17)
|
NCLOY |
26.44 |
28.77 |
2.33 |
| 5 |
Filiault, Sophia K (16)
|
NCLOY |
27.03 |
28.85 |
1.82 |
| 6 |
Johnson, Caroline G (16)
|
NCLOY |
28.10 |
29.90 |
1.80 |
| 7 |
Ratcliff, Charlotte A (15)
|
NCLOY |
29.63 |
30.49 |
0.86 |
| 8 |
Breeden, Abbey (16)
|
NCLOY |
29.91 |
30.76 |
0.85 |
| 9 |
Kluepfel, Valerie J (15)
|
NCUN |
29.12 |
31.68 |
2.56 |
| 10 |
Aldridge, Olivia A (15)
|
NCLOY |
29.73 |
32.05 |
2.32 |
|
Misener, Jacqueline M (16)
|
NCLOY |
25.83 |
NS |
- |