| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Younger, Cole C (17)
|
VACGBD |
50.60 |
51.94 |
1.34 |
| 2 |
Vlahos, Luke A (18)
|
NCTYDE |
51.25 |
52.58 |
1.33 |
| 3 |
Mateer, Cameron K (17)
|
VACGBD |
53.53 |
53.49 |
-0.04 |
| 4 |
Muench, Hunter D (17)
|
VACGBD |
54.08 |
54.44 |
0.36 |
| 5 |
Muench, Cooper D (16)
|
VACGBD |
52.45 |
54.82 |
2.37 |
| 6 |
Rock, Noah L (15)
|
NCGCY |
52.93 |
55.47 |
2.54 |
| 7 |
McDonald, Logan J (15)
|
NCTYDE |
55.44 |
55.49 |
0.05 |
| 8 |
Womble, Ethan V (17)
|
NCGCY |
54.65 |
55.71 |
1.06 |
| 9 |
Smith, Sean C (16)
|
NCTYDE |
55.88 |
56.16 |
0.28 |
| 10 |
Nario, Austin P (17)
|
NCTYDE |
54.86 |
56.43 |
1.57 |
| 11 |
Insixiengmay, Steven (15)
|
NCTYDE |
58.31 |
57.02 |
-1.29 |
| 12 |
Valliere, Alexander H (15)
|
NCTYDE |
57.91 |
57.16 |
-0.75 |
| 13 |
Harris, Dax C (15)
|
NCGCY |
54.30 |
57.47 |
3.17 |
| 14 |
Tars, Robert M (16)
|
NCGCY |
56.64 |
57.82 |
1.18 |
| 15 |
Wyatt, James T (17)
|
NCTYDE |
54.40 |
58.08 |
3.68 |
| 16 |
Parks, Victor Z (15)
|
VAWAC |
58.76 |
59.02 |
0.26 |
| 17 |
Rouzie, Bryce T (15)
|
VACGBD |
1:00.39 |
59.94 |
-0.45 |
| 18 |
McKenna, Patrick J (17)
|
NCTYDE |
58.35 |
1:00.61 |
2.26 |
| 19 |
Rouzie, Blake A (17)
|
VACGBD |
1:00.90 |
1:01.05 |
0.15 |
| 20 |
Sever, Cooper A (15)
|
NCTWA |
59.76 |
1:01.12 |
1.36 |
| 21 |
Fore, Daniel G (15)
|
NCTYDE |
57.77 |
1:01.38 |
3.61 |
| 22 |
Miroshnichenko, Alexander A (16)
|
NCGCY |
57.13 |
1:01.48 |
4.35 |
| 23 |
Chaikin, Andrew E (17)
|
VAWAC |
58.03 |
1:02.09 |
4.06 |
| 24 |
Esposito, William P (16)
|
NCGCY |
1:00.00 |
1:02.74 |
2.74 |
| 25 |
McGuire, Dylan J (17)
|
NCTYDE |
1:00.98 |
1:02.91 |
1.93 |
| 26 |
Farrell, Michael E (15)
|
NCTYDE |
1:01.91 |
1:03.60 |
1.69 |
| 27 |
Smelzer, Luke R (15)
|
NCSGSA |
1:04.53 |
1:04.93 |
0.40 |
| 28 |
Rock, Owen G (17)
|
NCGCY |
1:01.10 |
1:06.62 |
5.52 |
| 29 |
Persson, Brodie A (15)
|
VACGBD |
|
1:11.12 |
- |
| 30 |
Hipps, Tigue J (15)
|
NCGCY |
1:15.40 |
1:17.34 |
1.94 |
|
Zhang, Ray (16)
|
NCGCY |
1:06.69 |
DQ |
- |