| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Perez, Brianna M (12)
|
MRNYSA |
4:58.30 |
5:00.37 |
2.07 |
| 2 |
Downes, Taylor P (12)
|
VAUN-3 |
5:07.17 |
5:06.77 |
-0.40 |
| 3 |
Furbay, Caroline D (12)
|
NCUN-7 |
5:14.14 |
5:10.00 |
-4.14 |
| 4 |
Pawa, Anika (11)
|
NCEAC |
5:18.56 |
5:13.44 |
-5.12 |
| 5 |
Bottari, Madeline E (12)
|
VAUN-3 |
5:20.09 |
5:17.99 |
-2.10 |
| 6 |
Stinnett, Sophia G (11)
|
NCHAC |
5:15.88 |
5:19.47 |
3.59 |
| 7 |
Fernando, Scarlett O (11)
|
NCEAC |
5:34.94 |
5:29.09 |
-5.85 |
| 8 |
McCrimmon, Quinn A (12)
|
NCHAC |
6:00.99 |
5:38.23 |
-22.76 |
| 9 |
Steele, Eliana C (12)
|
NCWOW |
6:00.00 |
5:43.37 |
-16.63 |
| 10 |
Weigand, Kelsey C (11)
|
NCWOW |
6:22.60 |
5:44.16 |
-38.44 |
| 11 |
Robertson, Catherine L (11)
|
VAUN-3 |
6:02.86 |
5:52.00 |
-10.86 |
| 12 |
Stevens, Jenna L (11)
|
VAUN-3 |
6:14.63 |
5:54.08 |
-20.55 |
| 13 |
Metzinger, Mya Z (12)
|
NCWOW |
6:00.00 |
5:57.79 |
-2.21 |
| 14 |
Reaves, Camren G (11)
|
NCWOW |
5:58.52 |
6:03.78 |
5.26 |
| 15 |
Greaves, London C (12)
|
NCNEAL |
|
6:25.79 |
- |
| 16 |
Mondul, Angelia R (11)
|
VADYST |
|
6:50.24 |
- |