| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Matthews, Margaret J (7)
|
NCSTAR |
|
26.12 |
- |
| 2 |
Wolfe, Meira A (8)
|
NCSFSC |
29.25 |
28.37 |
-0.88 |
| 3 |
von der Lippe, Virginia F (7)
|
NCSTAR |
|
29.90 |
- |
| 4 |
Cirigliano, Lucia M (7)
|
NCSFSC |
29.65 |
30.29 |
0.64 |
| 5 |
Colon, Leah P (8)
|
NCEAC |
37.60 |
34.67 |
-2.93 |
| 6 |
Haislip, Riley G (7)
|
NCSFSC |
|
39.79 |
- |
| 7 |
McDonald, Kirby D (8)
|
NCSFSC |
35.18 |
41.36 |
6.18 |
|
Allgood, Carter G (7)
|
NCEAC |
45.45 |
DQ |
- |
|
Brevorka-Wells, Caroline M (7)
|
NCEAC |
49.69 |
DQ |
- |
|
Markovich, Holly J (7)
|
NCEAC |
53.56 |
DQ |
- |
|
Murphy, Natalie A (7)
|
NCSFSC |
|
DQ |
- |
|
Rumley, Parker K (7)
|
NCSTAR |
|
NS |
- |