| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Babel, Lilly Q (14)
|
NCWOW |
2:55.46 |
2:51.65 |
-3.81 |
| 2 |
Williams, Natalie A (14)
|
NCSSS |
2:48.82 |
2:55.27 |
6.45 |
| 3 |
Temple, Margaret B (14)
|
NCHCAC |
2:56.30 |
2:57.48 |
1.18 |
| 4 |
Cornell, Meghan R (13)
|
NCHCAC |
2:57.07 |
2:59.27 |
2.20 |
| 5 |
Taylor, Alyson M (14)
|
NCECA |
|
3:05.34 |
- |
| 6 |
Yoder, Anna K (14)
|
NCECA |
|
3:12.57 |
- |
| 7 |
Wood, Haley J (14)
|
NCWOW |
3:19.20 |
3:15.85 |
-3.35 |
|
Oliveira, Adelaide B (13)
|
NCBAC |
3:26.31 |
NS |
- |
|
Vanderkooi, Lily C (13)
|
NCECA |
|
NS |
- |
|
Luckey, Ashlynn G (13)
|
NCRMY |
4:04.20 |
NS |
- |
|
Everhart, Madeline G (14)
|
NCSSS |
3:13.74 |
NS |
- |