| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Han, Katherine (11)
|
AMKW |
2:31.32 |
2:25.55 |
-5.77 |
| 2 |
DiMaio, Lyla A (12)
|
AMRGSY |
2:27.87 |
2:27.22 |
-0.65 |
| 3 |
Khanolkar, Mia S (11)
|
AMPTSC |
2:35.73 |
2:30.81 |
-4.92 |
| 4 |
Bookwalter, Allison G (11)
|
AMSVSC |
2:33.82 |
2:36.02 |
2.20 |
| 5 |
Turner, Alyssa J (11)
|
AM3RVS |
2:42.29 |
2:43.80 |
1.51 |
| 6 |
Jerez, Arianna M (12)
|
AMKW |
3:08.96 |
2:46.05 |
-22.91 |
| 7 |
Ziegler, Ella E (12)
|
AMRXA |
2:56.36 |
2:55.85 |
-0.51 |
| 8 |
Agens, Gemma M (12)
|
AMKW |
2:45.02 |
2:58.87 |
13.85 |
| 9 |
Alexander, Annabelle E (11)
|
AMSVSC |
3:40.25 |
3:19.15 |
-21.10 |
| 10 |
Cawley, Elizabeth B (11)
|
AMKW |
3:02.57 |
3:19.97 |
17.40 |
|
Plank, Myka G (11)
|
AMSRST |
2:42.35 |
DQ |
- |