| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kunze, Sarah A (10)
|
NIPACK |
30.43 |
29.79 |
-0.64 |
| 2 |
Crowley, Lila M (10)
|
NIGRSC |
35.52 |
34.81 |
-0.71 |
| 3 |
O'Brien, Jolie L (10)
|
NIWEBS |
33.45 |
34.96 |
1.51 |
| 4 |
Conner, Haley M (9)
|
NIGRAT |
37.54 |
37.04 |
-0.50 |
| 5 |
Mastrosimone, Nina A (10)
|
NIWEBS |
36.58 |
37.19 |
0.61 |
| 6 |
Carey, Lilly E (9)
|
NIVICT |
40.11 |
41.39 |
1.28 |
| 7 |
Schneider, Brooke J (10)
|
NIMERC |
46.77 |
44.79 |
-1.98 |
| 8 |
Agostini, Ava E (10)
|
NIGRSC |
48.99 |
46.81 |
-2.18 |
| 9 |
Brett, Catherine M (9)
|
NIPACK |
47.52 |
47.10 |
-0.42 |
| 10 |
Bermudez, Airianna L (10)
|
NIGRSC |
53.31 |
47.80 |
-5.51 |
| 11 |
Knittle, Kiley F (9)
|
NIGCOM |
50.98 |
48.95 |
-2.03 |
| 12 |
Ugalde, Susana G (9)
|
NIGRAT |
|
49.67 |
- |
| 13 |
Cosner, Brynn S (10)
|
NIGRSC |
50.57 |
50.67 |
0.10 |
| 14 |
Ricci, Elizabeth M (9)
|
NIVICT |
46.20 |
52.78 |
6.58 |
| 15 |
Lamphere, Carolyn L (9)
|
NIGCOM |
57.92 |
57.76 |
-0.16 |
|
Wing, Gianna I (10)
|
NIGRSC |
42.44 |
NS |
- |