| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Staley, Catherine E (11)
|
NIMERC |
29.47 |
29.65 |
0.18 |
| 2 |
Onze, Lauren W (11)
|
NIVICT |
31.75 |
30.56 |
-1.19 |
| 3 |
Share, Elizabeth A (12)
|
NIPMSC |
30.87 |
30.60 |
-0.27 |
| 4 |
Beutner, Ella R (12)
|
NIVICT |
30.10 |
30.94 |
0.84 |
| 5 |
Schwandt, Natalie K (11)
|
NIGRAT |
32.29 |
31.80 |
-0.49 |
| 6 |
Bedford, Marin B (11)
|
NIWEBS |
31.16 |
32.19 |
1.03 |
| 7 |
Kuhlkin, Elizabeth C (12)
|
NIGCOM |
34.63 |
32.41 |
-2.22 |
| 8 |
Richards, Sydney R (11)
|
NIGRAT |
32.73 |
32.52 |
-0.21 |
| 9 |
Anthony, Jillian M (11)
|
NIPMSC |
32.02 |
32.58 |
0.56 |
| 10 |
Resch, Rebecka J (11)
|
NIGRSC |
35.15 |
33.74 |
-1.41 |
| 11 |
Wojtas, Katherine A (11)
|
NIWEBS |
34.50 |
34.01 |
-0.49 |
| 12 |
Strausser, Cadence A (11)
|
NIGRAT |
35.17 |
35.35 |
0.18 |
| 13 |
Brett, Erin E (11)
|
NIPACK |
34.58 |
35.59 |
1.01 |
| 14 |
Thurston, Lauren A (12)
|
NIGCOM |
37.47 |
36.59 |
-0.88 |
| 15 |
Saysamone, Leina H (12)
|
NIGCOM |
39.80 |
37.58 |
-2.22 |
| 16 |
Grigg, Charlotte M (11)
|
NIGRSC |
37.95 |
37.78 |
-0.17 |
| 17 |
Unawane, Siya S (11)
|
NIGCOM |
40.22 |
38.21 |
-2.01 |
| 18 |
Marsh, Emma L (11)
|
NIGRSC |
43.82 |
38.90 |
-4.92 |
| 19 |
Downey, Jenna A (11)
|
NINEWW |
40.89 |
40.21 |
-0.68 |
| 20 |
Ugalde, Judith N (12)
|
NIGRAT |
|
41.24 |
- |