| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Gabrielle M (10)
|
NCGG |
33.03 |
32.62 |
-0.41 |
| 2 |
Chavez, Sophia Mae M (10)
|
NCAQTC |
35.37 |
33.41 |
-1.96 |
| 3 |
Wasson, Hannah L (8)
|
NCCVAC |
35.23 |
34.11 |
-1.12 |
| 4 |
Lopez-Rodriquez, Neyli M (10)
|
NCMSC |
36.43 |
35.62 |
-0.81 |
| 5 |
McNeal, Ella J (10)
|
NCGG |
38.19 |
36.89 |
-1.30 |
| 6 |
Baucom, Skylar G (10)
|
NCWA |
37.29 |
36.99 |
-0.30 |
| 7 |
Wilborn, Margaret M (10)
|
NCAQTC |
43.27 |
37.53 |
-5.74 |
| 8 |
Thomas, Phoebe G (10)
|
NCGG |
39.61 |
38.32 |
-1.29 |
| 9 |
Pfeifer, Emma E (10)
|
NCCVAC |
|
38.56 |
- |
| 10 |
Schiffel, Karsen L (9)
|
NCCVAC |
38.25 |
39.18 |
0.93 |
| 11 |
Purser, Maezie G (9)
|
NCWA |
|
41.31 |
- |
| 12 |
Robinson, Kennedi M (9)
|
NCGG |
|
41.81 |
- |
| 13 |
Handy, Elsie R (9)
|
NCCVAC |
51.38 |
47.30 |
-4.08 |
| 14 |
Suber, Eva C (9)
|
NCGG |
50.46 |
48.92 |
-1.54 |
| 15 |
Lail, Aubrey F (9)
|
NCCVAC |
|
1:27.70 |
- |