| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bangs, Kahlan M (10)
|
NCCVAC |
38.08 |
35.98 |
-2.10 |
| 2 |
Sic, Ana N (10)
|
NCMSC |
39.71 |
36.61 |
-3.10 |
| 3 |
Millsaps, Katherine G (7)
|
NCCVAC |
37.16 |
38.52 |
1.36 |
| 4 |
McGrath, Sarah A (10)
|
NCGG |
44.75 |
39.35 |
-5.40 |
| 5 |
Griffith, Isabella M (10)
|
NCLENR |
40.00 |
39.56 |
-0.44 |
| 6 |
Loomis, Ella R (10)
|
NCMSC |
|
40.60 |
- |
| 7 |
Lowman, Addison K (10)
|
NCUN |
|
40.66 |
- |
| 8 |
Huffman, Madison L (9)
|
NCCVAC |
42.40 |
40.96 |
-1.44 |
| 9 |
Holland, Triniti M (9)
|
NCWA |
39.79 |
41.13 |
1.34 |
| 10 |
Appling, Jocelyn C (10)
|
NCRCST |
40.03 |
41.55 |
1.52 |
| 11 |
Scheller, Kylie M (10)
|
NCCVAC |
1:02.28 |
1:01.31 |
-0.97 |
|
Gibson, Kaelyn M (10)
|
NCCVAC |
48.61 |
NS |
- |
|
Jones, Lucy E (9)
|
NCMSC |
45.99 |
NS |
- |