| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rose, Sydney S (12)
|
NCCVAC |
31.56 |
29.66 |
-1.90 |
| 2 |
Hedrick, Chloe M (12)
|
NCCVAC |
30.80 |
30.67 |
-0.13 |
| 3 |
Miller, Zoie C (12)
|
NCLENR |
38.48 |
30.98 |
-7.50 |
| 4 |
Huffman, Sydney M (12)
|
NCCVAC |
30.98 |
31.00 |
0.02 |
| 5 |
Yercheck, Gabrielle E (12)
|
NCWA |
31.91 |
32.03 |
0.12 |
| 6 |
Matheney, Kenna J (12)
|
NCGG |
28.70 |
32.13 |
3.43 |
| 7 |
Aragon-Zamora, Angelica (12)
|
NCCVAC |
33.20 |
32.18 |
-1.02 |
| 8 |
Clontz, Leah J (12)
|
NCCVAC |
31.78 |
32.63 |
0.85 |
| 9 |
Rautiola, Larissa L (12)
|
NCCVAC |
32.60 |
32.85 |
0.25 |
| 10 |
Lowe, Cassidy M (12)
|
NCLENR |
33.02 |
33.08 |
0.06 |
| 11 |
Rousey, Margaret A (11)
|
NCCVAC |
36.13 |
34.18 |
-1.95 |
| 12 |
Thornburg, Emma G (11)
|
NCGG |
35.16 |
34.39 |
-0.77 |
| 13 |
Rodriguez, Keyla L (12)
|
NCMSC |
36.38 |
35.31 |
-1.07 |
| 14 |
Carswell, Kennedy G (12)
|
NCMSC |
46.01 |
35.83 |
-10.18 |
| 15 |
Beltran-Trejo, Nicole (11)
|
NCCVAC |
36.89 |
36.27 |
-0.62 |
| 16 |
Canipe, Libby (11)
|
NCSAC |
36.85 |
36.49 |
-0.36 |
| 17 |
Allen, Jessica (11)
|
NCMSC |
36.39 |
36.53 |
0.14 |
| 18 |
Wall, Olivia A (11)
|
NCWA |
39.24 |
36.81 |
-2.43 |
| 19 |
Schlageter, Jordan A (12)
|
NCLENR |
39.60 |
37.70 |
-1.90 |
| 20 |
Olvera, Karina (11)
|
NCCVAC |
39.53 |
40.43 |
0.90 |
| 21 |
Cullen, Riley W (11)
|
NCMSC |
43.64 |
40.51 |
-3.13 |
| 22 |
Murphy, Riley J (11)
|
NCLENR |
39.31 |
40.77 |
1.46 |
| 23 |
Foor, Elizabeth G (12)
|
NCGG |
|
41.37 |
- |
| 24 |
Lugo, Veronica R (11)
|
NCRCST |
|
42.95 |
- |
| 25 |
Wentz, Elizabeth R (12)
|
NCWA |
35.29 |
43.16 |
7.87 |
| 26 |
Stafford, Makenna L (12)
|
NCCVAC |
44.05 |
43.30 |
-0.75 |
| 27 |
Davison, Lillian M (11)
|
NCGG |
42.73 |
43.85 |
1.12 |
| 28 |
Helderman, Maggie L (12)
|
NCLENR |
54.23 |
49.87 |
-4.36 |
|
Ferrell, Abby L (12)
|
NCGG |
|
NS |
- |