| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Terhardt, Kayla A (12)
|
NCRCST |
31.78 |
31.19 |
-0.59 |
| 2 |
Alvarez, Dayana N (12)
|
NCCVAC |
35.37 |
32.87 |
-2.50 |
| 3 |
Robinson, Lillian B (11)
|
NCGG |
34.11 |
33.84 |
-0.27 |
| 4 |
Kohler, Madison N (11)
|
NCWA |
34.71 |
33.85 |
-0.86 |
| 5 |
Seraz, Larissa F (12)
|
NCCVAC |
37.84 |
34.30 |
-3.54 |
| 6 |
Jones, Reilly E (12)
|
NCWA |
38.20 |
34.89 |
-3.31 |
| 7 |
Finney, Merrill P (12)
|
NCCVAC |
40.73 |
35.48 |
-5.25 |
| 8 |
Schiffel, Karsen L (11)
|
NCCVAC |
34.36 |
35.56 |
1.20 |
| 9 |
Pfeifer, Emma E (11)
|
NCCVAC |
35.58 |
38.23 |
2.65 |
| 10 |
Pavlinsky, Adaline E (12)
|
NCCVAC |
|
38.94 |
- |
| 11 |
Pintuff, Jenna R (11)
|
NCRCST |
42.05 |
41.92 |
-0.13 |
| 13 |
Short, Addison L (11)
|
NCRCST |
53.48 |
44.58 |
-8.90 |
| 14 |
Mendoza Perez, Erica E (12)
|
NCMSC |
|
49.07 |
- |
| 15 |
Roper, Emma K (11)
|
NCMSC |
|
1:06.35 |
- |