| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hedrick, Noah A (11)
|
NCCVAC |
31.17 |
30.01 |
-1.16 |
| 2 |
Carswell, Nathaniel R (12)
|
NCMSC |
31.07 |
31.38 |
0.31 |
| 3 |
Tabor, Noah E (11)
|
NCGG |
30.66 |
33.18 |
2.52 |
| 4 |
Olguin-Abreu, David F (12)
|
NCCVAC |
35.74 |
33.45 |
-2.29 |
| 5 |
Kesterson, Christopher T (12)
|
NCRCST |
35.32 |
33.68 |
-1.64 |
| 6 |
Brantley, Landon N (11)
|
NCWA |
49.20 |
40.63 |
-8.57 |
| 7 |
Moseley, Josiah L (12)
|
NCCVAC |
52.09 |
42.93 |
-9.16 |
| 8 |
Ramirez, Daniel A (12)
|
NCCVAC |
46.94 |
43.44 |
-3.50 |
| 9 |
Roper, Eli J (11)
|
NCMSC |
|
1:03.18 |
- |
|
Major, Austin L (12)
|
NCGG |
32.20 |
NS |
- |
|
Cantu, Damian (11)
|
NCRCST |
46.96 |
NS |
- |