| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Gunner L (12)
|
NCCVAC |
29.42 |
28.32 |
-1.10 |
| 2 |
Hedrick, Noah A (12)
|
NCCVAC |
29.62 |
29.53 |
-0.09 |
| 3 |
Kines, Graham H (12)
|
NCGG |
30.89 |
30.12 |
-0.77 |
| 4 |
Makdad, Michael G (11)
|
NCWST |
32.03 |
31.42 |
-0.61 |
| 5 |
Olguin-Abreu, David F (12)
|
NCCVAC |
33.45 |
33.72 |
0.27 |
| 6 |
Kesterson, Christopher T (12)
|
NCRCST |
33.68 |
35.38 |
1.70 |
| 7 |
Brantley, Landon N (12)
|
NCWA |
40.63 |
43.00 |
2.37 |
| 8 |
Whisnant, Boone M (11)
|
NCAQTC |
45.40 |
44.96 |
-0.44 |
| 9 |
Moseley, Josiah L (12)
|
NCCVAC |
42.93 |
45.38 |
2.45 |
| 10 |
Denny, Kole W (12)
|
NCWST |
|
46.22 |
- |
| 11 |
Irish, Aiden D (11)
|
NCGG |
47.54 |
46.69 |
-0.85 |
| 12 |
Denny, Keelan C (12)
|
NCWST |
|
50.35 |
- |
|
Cantu, Damian (11)
|
NCRCST |
40.07 |
NS |
- |