| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lynch, Kirsten N (12)
|
NCGG |
34.72 |
37.64 |
2.92 |
| 2 |
Schrock, Annalee K (11)
|
NCWA |
39.22 |
37.79 |
-1.43 |
| 3 |
Lee, Jovie M (11)
|
NCCVAC |
39.72 |
37.90 |
-1.82 |
| 4 |
Olguin-Abreu, Denise (12)
|
NCCVAC |
39.37 |
39.62 |
0.25 |
| 5 |
Kale, Zelina M (12)
|
NCCVAC |
39.01 |
40.19 |
1.18 |
| 6 |
Caudle, Riley S (12)
|
NCCVAC |
41.20 |
40.35 |
-0.85 |
| 7 |
Stewart, Leah M (11)
|
NCCVAC |
42.16 |
41.24 |
-0.92 |
| 8 |
Li, Rui T (11)
|
NCAQTC |
|
44.52 |
- |
| 9 |
Van Meter, Claire V (11)
|
NCCVAC |
43.76 |
44.98 |
1.22 |
| 10 |
Carroll, Kiersten I (12)
|
NCCVAC |
|
45.94 |
- |
| 11 |
Alvarez, Dayana N (11)
|
NCCVAC |
1:00.73 |
46.86 |
-13.87 |
| 12 |
Alvarado-Gamborino, Miranda (12)
|
NCMSC |
|
47.32 |
- |
| 13 |
Greene, Jordyn A (11)
|
NCMSC |
50.11 |
49.08 |
-1.03 |
| 14 |
Floyd, Lilly G (11)
|
NCGG |
47.52 |
50.97 |
3.45 |
| 15 |
Seraz, Larissa F (11)
|
NCCVAC |
1:10.73 |
51.15 |
-19.58 |
| 16 |
Negrete-Perez, Stephanie (12)
|
NCCVAC |
53.92 |
54.16 |
0.24 |
| 17 |
Ramirez-Soto, Vanessa (12)
|
NCCVAC |
|
56.60 |
- |
| 18 |
White, Skyler R (11)
|
NCCVAC |
1:03.85 |
58.50 |
-5.35 |