| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Claborn, Alyssa A (12)
|
NCLOY |
28.99 |
28.97 |
-0.02 |
| 2 |
Stinnett, Sophia G (12)
|
NCHAC |
31.57 |
31.12 |
-0.45 |
| 3 |
Spreitzer, Aubree K (12)
|
NCCAT |
32.26 |
32.18 |
-0.08 |
| 4 |
Burleigh, Sydney R (12)
|
NCCKS |
33.55 |
32.44 |
-1.11 |
| 5 |
Roberts, Grace S (12)
|
NCCAT |
31.35 |
32.80 |
1.45 |
| 6 |
Palmer, Caroline L (12)
|
NCCAT |
34.74 |
34.20 |
-0.54 |
| 7 |
Wiechers, Abigail F (12)
|
NCLOY |
33.79 |
34.25 |
0.46 |
| 8 |
Thornburg, Sophie J (11)
|
NCCAT |
33.20 |
34.47 |
1.27 |
| 9 |
Walker, LaiCie Mei R (11)
|
VADYST |
33.58 |
34.74 |
1.16 |
| 10 |
White, Piper C (12)
|
NCHAC |
35.35 |
35.71 |
0.36 |
| 11 |
Murray, Kayla R (12)
|
NCLOY |
34.10 |
36.05 |
1.95 |
| 12 |
Komelasky, Sydney M (11)
|
NCCKS |
38.49 |
36.54 |
-1.95 |
| 13 |
Jimenez-Reller, Esperanza (12)
|
NCHAC |
36.86 |
37.22 |
0.36 |
| 14 |
Camensky, Sarah E (11)
|
NCLOY |
36.96 |
38.13 |
1.17 |
| 15 |
Pechuekonis, Nikole M (12)
|
NCLOY |
38.29 |
38.23 |
-0.06 |
| 16 |
Jones, Jessica E (12)
|
NCLOY |
37.53 |
39.44 |
1.91 |
| 17 |
Goin, Ella R (12)
|
NCCAT |
39.61 |
39.46 |
-0.15 |
| 18 |
Stacy, Kassidy L (12)
|
NCLOY |
42.36 |
43.97 |
1.61 |
| 19 |
Siler, Kaitlyn G (11)
|
NCCKS |
50.33 |
48.80 |
-1.53 |
| 20 |
Camensky, Emily M (11)
|
NCLOY |
46.45 |
50.92 |
4.47 |
| 21 |
Ropchack, Evie A (11)
|
NCLOY |
49.73 |
51.32 |
1.59 |