| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sangasy, KhaNy K (12)
|
NIGCOM |
29.05 |
28.74 |
-0.31 |
| 2 |
Bouwens, Emma L (11)
|
NIHAST |
32.99 |
31.93 |
-1.06 |
| 3 |
Smolinski, Evelynn J (11)
|
NIHAST |
35.69 |
34.25 |
-1.44 |
| 4 |
Kelley, Michaela G (11)
|
NIGRSC |
35.71 |
35.23 |
-0.48 |
| 5 |
Stonehouse, Sierra L (12)
|
NIGRSC |
37.09 |
35.92 |
-1.17 |
| 6 |
Resch, Rebecka J (11)
|
NIGRSC |
35.15 |
38.52 |
3.37 |
| 7 |
Rozalski, Jasmine L (12)
|
NIMERC |
37.30 |
38.56 |
1.26 |
| 8 |
Greisch, Alisha D (11)
|
NIGRSC |
38.45 |
38.93 |
0.48 |
| 9 |
Stefanou, Ava C (11)
|
NIQUIK |
37.53 |
39.23 |
1.70 |
| 10 |
Kenny, Elisabeth A (12)
|
NIQUIK |
39.74 |
39.78 |
0.04 |
| 11 |
Rouse, Ellie R (12)
|
NIGRAT |
38.65 |
40.16 |
1.51 |
| 12 |
Cabral, Aurora (12)
|
NIRRST |
|
41.11 |
- |
| 13 |
Farina, Samantha R (11)
|
NIGRSC |
41.84 |
41.13 |
-0.71 |
| 14 |
Stoos, Arianna E (11)
|
NIGRAT |
41.59 |
43.24 |
1.65 |
| 15 |
Wilson, Taylor J (11)
|
NIHAST |
|
44.81 |
- |
|
Delehanty, Maeve K (11)
|
NIRRST |
40.52 |
DQ |
- |
|
Osborne, Samantha R (12)
|
NIGRSC |
36.21 |
NS |
- |
|
Rist, Olivia B (11)
|
NIHAST |
51.70 |
NS |
- |