| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Delehanty, Maeve K (11)
|
NIRRST |
38.00 |
36.89 |
-1.11 |
| 2 |
Bouwens, Emma L (11)
|
NIHAST |
38.45 |
37.52 |
-0.93 |
| 3 |
Smolinski, Evelynn J (11)
|
NIHAST |
42.78 |
40.62 |
-2.16 |
| 4 |
Greisch, Alisha D (11)
|
NIGRSC |
42.29 |
42.00 |
-0.29 |
| 5 |
Kelley, Michaela G (11)
|
NIGRSC |
44.54 |
45.22 |
0.68 |
| 6 |
Stonehouse, Sierra L (12)
|
NIGRSC |
47.18 |
45.25 |
-1.93 |
| 7 |
Kenny, Elisabeth A (12)
|
NIQUIK |
43.92 |
45.29 |
1.37 |
| 8 |
Resch, Rebecka J (11)
|
NIGRSC |
42.34 |
45.63 |
3.29 |
| 9 |
Rouse, Ellie R (12)
|
NIGRAT |
42.94 |
46.77 |
3.83 |
| 10 |
Stefanou, Ava C (11)
|
NIQUIK |
54.55 |
48.58 |
-5.97 |
| 11 |
Stoos, Arianna E (11)
|
NIGRAT |
47.50 |
53.03 |
5.53 |
| 12 |
Farina, Samantha R (11)
|
NIGRSC |
48.81 |
53.70 |
4.89 |
| 13 |
Cabral, Aurora (12)
|
NIRRST |
55.74 |
53.87 |
-1.87 |
| 14 |
Wilson, Taylor J (11)
|
NIHAST |
|
1:00.51 |
- |
|
Rist, Olivia B (11)
|
NIHAST |
53.09 |
NS |
- |
|
Osborne, Samantha R (12)
|
NIGRSC |
44.40 |
NS |
- |