| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stapleton, Tallis M (8)
|
GUUNAT |
39.52 |
37.77 |
-1.75 |
| 2 |
Pham, Allyson (8)
|
GUCFSC |
42.73 |
41.59 |
-1.14 |
| 3 |
Loftin, Grace W (8)
|
GUVSST |
|
46.00 |
- |
| 4 |
Pacoma, Kaylee Angela D (7)
|
GUCFSC |
48.94 |
49.02 |
0.08 |
| 5 |
Nasr, Isabella Y (7)
|
GUCFSC |
58.74 |
50.60 |
-8.14 |
| 6 |
Nelson, Lauren O (8)
|
GUSPA |
|
50.81 |
- |
| 7 |
Heredia, Amanda (8)
|
GUCFSC |
50.62 |
51.77 |
1.15 |
| 8 |
Olson, Ingrid E (7)
|
GUUN-C |
51.35 |
52.91 |
1.56 |
| 9 |
Maddan, Molly Kate K (7)
|
GUCFSC |
54.23 |
53.10 |
-1.13 |
| 9 |
Lucas, Emma J (6)
|
GUVSST |
|
53.10 |
- |
| 11 |
Tran, Emma N (8)
|
GUCFSC |
|
53.79 |
- |
| 12 |
Clavette, Sophia J (8)
|
GUCFSC |
|
54.27 |
- |
| 13 |
Cui, Dina (8)
|
GUCFSC |
1:01.98 |
56.54 |
-5.44 |
| 14 |
Akinjaiyeju, Fiayoshola G (8)
|
GUSPA |
1:00.59 |
57.44 |
-3.15 |
| 15 |
Jasme, Mia (8)
|
GUCFSC |
|
58.38 |
- |
| 16 |
Rogojina, Kaitlyn G (6)
|
GUCFSC |
1:01.86 |
59.01 |
-2.85 |
| 17 |
Montague, Quinn M (7)
|
GUCFSC |
57.98 |
1:01.55 |
3.57 |
| 18 |
Caver, Laila O (7)
|
GUSPA |
1:03.01 |
1:01.65 |
-1.36 |
| 19 |
Betts, Mia K (5)
|
GUCFSC |
1:10.08 |
1:02.57 |
-7.51 |
| 20 |
Kim, Soovin (8)
|
GUCFSC |
|
1:03.00 |
- |
| 21 |
Rajendran, Tanvi V (8)
|
GUCFSC |
1:04.66 |
1:04.22 |
-0.44 |
| 22 |
Svoboda, Maryanne C (7)
|
GUCFSC |
1:03.15 |
1:07.04 |
3.89 |
| 23 |
Brown, Sarah E (8)
|
GUCFSC |
|
1:07.76 |
- |
| 24 |
Flosser, Audrey H (6)
|
GUSPA |
|
1:08.09 |
- |
|
Caver, Lauren A (6)
|
GUSPA |
1:19.93 |
DQ |
- |
|
Ramsey, Laken M (8)
|
GUCFSC |
|
DQ |
- |
|
Chen, Yan Ning (6)
|
GUVSST |
56.02 |
NS |
- |
|
Nguyen, Alyana Q (7)
|
GUCFSC |
1:32.52 |
NS |
- |