| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yang, Chloe Y (8)
|
GUSSAN |
23.61 |
22.45 |
-1.16 |
| 2 |
Villarreal, Addyson G (8)
|
GUBTA |
23.42 |
22.75 |
-0.67 |
| 3 |
Hadley, Kaitlyn N (8)
|
GUSSAN |
26.38 |
26.32 |
-0.06 |
| 4 |
Armand, Ava G (8)
|
GUSSAN |
29.27 |
27.27 |
-2.00 |
| 5 |
Ehlers, Naomi G (8)
|
GUOFSC |
27.74 |
28.90 |
1.16 |
| 6 |
Dulworth, Ella M (8)
|
GUSSAN |
33.46 |
29.35 |
-4.11 |
| 7 |
Casperson, Cara E (8)
|
GUBTA |
30.57 |
29.41 |
-1.16 |
| 8 |
Kalland, Adelyn M (8)
|
GUBTA |
|
30.09 |
- |
| 9 |
Coker, Vivienne L (8)
|
GUSSAN |
31.03 |
30.73 |
-0.30 |
| 10 |
Walker, Elise M (8)
|
GUSSAN |
33.56 |
34.50 |
0.94 |
|
Winslow, Ava M (8)
|
GUBTA |
|
DQ |
- |
|
Mehra, Mehak S (8)
|
GUOFSC |
|
DQ |
- |
|
Morataya, Hazel E (8)
|
GUBTA |
|
NS |
- |
|
Chandrahasan, Eshana A (8)
|
GUOFSC |
|
NS |
- |