| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barnard, Marley R (12)
|
GUPFL |
2:52.13 |
2:47.30 |
-4.83 |
| 2 |
Rasmussen, Katherine (12)
|
GUAGS |
2:53.15 |
2:51.20 |
-1.95 |
| 3 |
Nikolakopoulos, Sofia A (12)
|
GUESCA |
2:58.24 |
2:53.79 |
-4.45 |
| 4 |
McNames, Gabriella G (12)
|
GUPACE |
2:38.33 |
2:54.50 |
16.17 |
| 5 |
Pham, Elizabeth A (12)
|
GUESCA |
3:02.40 |
2:59.53 |
-2.87 |
| 6 |
Corbin, Paige G (12)
|
GUPACK |
2:57.65 |
3:00.65 |
3.00 |
| 7 |
Buenemann, Jayden (12)
|
GUAGS |
3:20.02 |
3:05.20 |
-14.82 |
| 8 |
Loya, Ingrid I (11)
|
GUAGS |
3:12.67 |
3:05.47 |
-7.20 |
| 9 |
Madden, Jasmine J (11)
|
GURICE |
3:12.54 |
3:05.86 |
-6.68 |
| 10 |
Keggins, Peyton L (11)
|
GUPACK |
3:05.56 |
3:06.28 |
0.72 |
| 11 |
Makasheva, Kamila (11)
|
GURICE |
3:12.30 |
3:07.04 |
-5.26 |
| 12 |
Wells, Angela M (12)
|
GUKATY |
2:57.14 |
3:07.55 |
10.41 |
| 13 |
Wylie-Young, Hannah F (12)
|
GUPACK |
3:17.10 |
3:08.67 |
-8.43 |
| 14 |
Katz, Lilah R (11)
|
GUTEST |
3:09.10 |
3:09.27 |
0.17 |
| 15 |
Tremaine, Malea T (12)
|
GUESCA |
3:03.68 |
3:09.43 |
5.75 |
| 16 |
Rogers, Emilee E (12)
|
GUTEST |
3:11.44 |
3:09.74 |
-1.70 |
| 17 |
Baker, Brooke C (12)
|
GUESCA |
2:53.01 |
3:16.91 |
23.90 |
| 18 |
Murray, Claire A (11)
|
GURICE |
3:27.09 |
3:18.77 |
-8.32 |
| 19 |
Gorham, Lillian M (12)
|
GUPFL |
3:07.23 |
3:19.52 |
12.29 |
| 20 |
Londono, Caylee R (11)
|
GUSPA |
3:22.42 |
3:21.61 |
-0.81 |
| 21 |
York, Emily A (11)
|
GURICE |
3:08.98 |
3:24.03 |
15.05 |
| 22 |
Williams, Mary O (12)
|
GUPFL |
3:01.29 |
3:24.18 |
22.89 |
| 23 |
Kamman, Finley S (12)
|
GURICE |
3:22.91 |
3:25.28 |
2.37 |
| 24 |
Hill, Britlynn M (12)
|
GUPFL |
3:02.63 |
3:27.60 |
24.97 |
| 25 |
Miller, Marinne L (11)
|
GUTEST |
3:17.39 |
3:30.26 |
12.87 |
| 26 |
Bergman, Taylor J (11)
|
GURICE |
3:04.30 |
3:34.47 |
30.17 |
| 27 |
Rajanna, Krisha (11)
|
GUKATY |
3:21.92 |
3:35.45 |
13.53 |
| 28 |
Beery, Daniela N (11)
|
GUPACK |
3:57.16 |
3:35.47 |
-21.69 |
|
Qi, Vanessa S (11)
|
GUKATY |
|
DQ |
- |