| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Longbottom, Charlotte V (17)
|
GUPACK |
2:48.60 |
2:48.57 |
-0.03 |
| 2 |
Vindiola, Madeline E (16)
|
GUPACK |
3:00.19 |
2:56.32 |
-3.87 |
| 3 |
Peng, Jessica (17)
|
GUAGS |
|
3:01.05 |
- |
| 4 |
Gottfried, Charlotte A (16)
|
GUPACK |
3:03.34 |
3:05.95 |
2.61 |
| 5 |
Ryan, Alyssa Q (15)
|
GUPACE |
3:27.24 |
3:06.88 |
-20.36 |
| 6 |
Berkman, Kaylie J (16)
|
GUPFL |
3:23.76 |
3:16.80 |
-6.96 |
| 7 |
Stewart, Grace I (15)
|
GUTEST |
3:09.22 |
3:18.29 |
9.07 |
| 8 |
Crawford, Cameron R (15)
|
GUPACK |
3:08.73 |
3:19.48 |
10.75 |
| 9 |
Lindsey, Kyla J (15)
|
GUPACE |
3:42.56 |
3:20.05 |
-22.51 |
| 10 |
Riddle, Allia M (15)
|
GUAGS |
3:27.33 |
3:28.46 |
1.13 |
| 11 |
Watts, Emma F (15)
|
GUPFL |
|
3:41.64 |
- |