| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ceraudo, Ava E (12)
|
GUDADS |
2:51.26 |
2:45.40 |
-5.86 |
| 2 |
Guerrero, Stacy X (11)
|
GUHCAP |
2:44.48 |
2:45.59 |
1.11 |
| 3 |
Powers, Brooke C (12)
|
GUDADS |
2:53.92 |
2:50.24 |
-3.68 |
| 4 |
Fischbach, Claire E (11)
|
GUDADS |
2:52.37 |
2:50.36 |
-2.01 |
| 5 |
Tremaine, Malea T (12)
|
GUESCA |
2:44.06 |
2:52.23 |
8.17 |
| 6 |
Pham, Elizabeth A (12)
|
GUESCA |
2:54.80 |
2:56.03 |
1.23 |
| 7 |
Clechenko, Lillian N (12)
|
GUDADS |
|
3:01.99 |
- |
| 8 |
Ly, Yvonne K (11)
|
GUESCA |
2:56.00 |
3:03.11 |
7.11 |
| 9 |
Tran, Emily H (11)
|
GUESCA |
3:06.37 |
3:03.43 |
-2.94 |
| 10 |
Tran, Victoria M (12)
|
GUSWAT |
3:07.08 |
3:05.59 |
-1.49 |
| 11 |
Baker, Brooke C (12)
|
GUESCA |
3:18.41 |
3:08.50 |
-9.91 |
| 12 |
Rodriguez, Gissele K (12)
|
GUHCAP |
3:07.58 |
3:12.07 |
4.49 |
| 13 |
Burke, Anna E (11)
|
GUPEAK |
3:20.70 |
3:13.02 |
-7.68 |
| 14 |
McManus, Taryn M (11)
|
GUPEAK |
3:15.59 |
3:13.82 |
-1.77 |
| 15 |
Garcia, Ariana E (12)
|
GUPEAK |
3:27.47 |
3:22.85 |
-4.62 |
| 16 |
Arreguin, Ayleen (11)
|
GUHCAP |
|
3:23.49 |
- |
| 17 |
Rogers, Sofie A (11)
|
GUPEAK |
3:45.40 |
3:38.83 |
-6.57 |
|
Astramskas, Ava L (11)
|
GUSWAT |
|
NS |
- |