| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hickok, Jackson J (10)
|
GUUN |
38.02 |
37.03 |
-0.99 |
| 2 |
Nelson, Jaxon (10)
|
GUUN |
41.88 |
39.44 |
-2.44 |
| 3 |
Vandertie, Crichton J (10)
|
GUUN |
38.13 |
39.93 |
1.80 |
| 4 |
Cashman, Cullen J (10)
|
NENE |
36.84 |
40.38 |
3.54 |
| 5 |
Zapata, Giovanie (10)
|
FLTV |
41.18 |
41.13 |
-0.05 |
| 6 |
Lopez, Micah (10)
|
ARTEAM |
38.67 |
41.42 |
2.75 |
| 7 |
Rodriguez, Gabrielpaul C (9)
|
GUUN |
41.56 |
41.61 |
0.05 |
| 8 |
Williams, Emory (10)
|
GUUN |
43.70 |
45.62 |
1.92 |
| 9 |
Samonte, Cameron T (10)
|
GULSST |
47.33 |
46.16 |
-1.17 |
| 10 |
Vedder, Thomas B (10)
|
NIUSTA |
47.60 |
46.29 |
-1.31 |
| 11 |
Duncan, Thomas (10)
|
ARTEAM |
1:01.00 |
47.10 |
-13.90 |
| 12 |
Bianchi, Kai (9)
|
FLFL |
47.02 |
47.41 |
0.39 |
| 13 |
Powell, Justin D (9)
|
NCHHST |
40.69 |
48.05 |
7.36 |
| 14 |
Arnstein, Ethan (10)
|
FLBA |
56.40 |
52.13 |
-4.27 |
| 15 |
Alder, Lincoln D (9)
|
GUUN |
1:03.19 |
52.40 |
-10.79 |
| 16 |
Evelsizer, Reese (9)
|
OZSMSC |
54.44 |
54.65 |
0.21 |
| 17 |
Carter, Garrett (10)
|
ARTEAM |
55.45 |
57.00 |
1.55 |
|
Spencer, Alistair B (9)
|
GUUN |
49.10 |
NS |
- |