| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kotch, Delaney S (10)
|
GUESA |
44.35 |
44.14 |
-0.21 |
| 2 |
Middlebrooks, Ella K (10)
|
GUNEHA |
49.06 |
47.37 |
-1.69 |
| 3 |
Tindall, Molly K (10)
|
GUESA |
48.69 |
47.69 |
-1.00 |
| 4 |
Malbrough, Nala L (10)
|
GUESA |
50.95 |
48.53 |
-2.42 |
| 5 |
Hebert, Lori L (10)
|
GUNEHA |
56.96 |
49.46 |
-7.50 |
| 6 |
Lee, Lydia (10)
|
GUUSA |
48.65 |
49.51 |
0.86 |
| 7 |
Fuentes-Pardo, Alejandra N (10)
|
GUESA |
52.48 |
51.51 |
-0.97 |
| 8 |
Carballo, Barbara B (10)
|
GUESA |
55.20 |
53.83 |
-1.37 |
| 9 |
Bellon, Addison R (10)
|
GUESA |
53.20 |
57.01 |
3.81 |
| 10 |
Quinteros, Emely D (10)
|
GUNEHA |
1:10.79 |
1:07.22 |
-3.57 |