| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wilson, Addison R (8)
|
NCWST |
42.29 |
39.43 |
-2.86 |
| 2 |
Wasson, Hannah L (8)
|
NCCVAC |
42.59 |
40.30 |
-2.29 |
| 3 |
Harmon, Elizabeth G (8)
|
NCATOM |
|
43.30 |
- |
| 4 |
Barrick, Riley D (7)
|
NCSAC |
|
44.85 |
- |
| 5 |
Bennett, Addison R (8)
|
NCRCST |
46.05 |
46.55 |
0.50 |
| 6 |
Mai, Jamie V (8)
|
NCATOM |
44.52 |
48.46 |
3.94 |
| 7 |
Robinson, Kennedi M (8)
|
NCGG |
51.70 |
50.08 |
-1.62 |
| 8 |
Willer, Avery L (8)
|
NCGG |
|
50.39 |
- |
| 9 |
Handy, Elsie R (8)
|
NCCVAC |
57.19 |
51.58 |
-5.61 |
| 10 |
Veera, Seehe (8)
|
NCNMA |
|
51.87 |
- |
| 11 |
Hill, Elise S (8)
|
NCNMA |
|
52.47 |
- |
| 12 |
Johnson, Valerie D (6)
|
NCCVAC |
|
52.55 |
- |
| 13 |
Miller, Brinley J (8)
|
NCNMA |
|
52.66 |
- |
| 14 |
Millsaps, Katherine G (6)
|
NCCVAC |
|
52.79 |
- |
| 15 |
Bullins, Blakeney E (8)
|
NCATOM |
1:08.72 |
55.35 |
-13.37 |
| 16 |
Huffman, Madison L (7)
|
NCCVAC |
|
1:00.44 |
- |
| 17 |
Stancil, Ellie G (7)
|
NCGG |
|
1:01.62 |
- |
| 18 |
Bartlett, Veronica D (7)
|
NCCVAC |
|
1:08.58 |
- |
| 19 |
Beltran-Trejo, Fernanda (6)
|
NCCVAC |
|
1:13.84 |
- |
| 20 |
Stawecki, Riley A (7)
|
NCLENR |
1:11.17 |
1:15.33 |
4.16 |
| 21 |
Cantu, Camila (8)
|
NCRCST |
|
1:20.29 |
- |