| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Drew, Kendell A (13)
|
AMMOON |
2:49.99 |
2:32.04 |
-17.95 |
| 2 |
Connors, Emily E (13)
|
AMMOON |
2:41.27 |
2:34.86 |
-6.41 |
| 3 |
Schroeder, Addison B (13)
|
AMMOON |
2:33.46 |
2:35.18 |
1.72 |
| 4 |
Zbezinski, Valerie M (17)
|
AMPRA |
3:01.47 |
2:39.43 |
-22.04 |
| 5 |
Bissett, Ainsley L (16)
|
AMPRA |
2:21.26 |
2:39.51 |
18.25 |
| 6 |
Smaglo, Paige M (14)
|
AMBWA |
3:10.16 |
2:43.88 |
-26.28 |
| 7 |
Connelly, Riley A (14)
|
AMPRA |
2:46.14 |
2:45.27 |
-0.87 |
| 8 |
Manczka, Madeline C (15)
|
AMPRA |
2:49.79 |
2:48.37 |
-1.42 |
| 9 |
Anderson, Ariana A (13)
|
AMPRA |
3:08.57 |
2:52.35 |
-16.22 |
| 10 |
Rowe, Emma E (14)
|
AMPRA |
3:20.35 |
3:09.58 |
-10.77 |
|
Carmazzi, Brittney A (17)
|
AMPRA |
2:04.48 |
NS |
- |
|
Cribbs, Mila E (14)
|
AMPRA |
2:29.40 |
NS |
- |
|
Smith, Megan A (16)
|
AMPRA |
2:29.32 |
NS |
- |