| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carlile, Austin J (10)
|
INFAST |
2:20.99 |
2:21.27 |
0.28 |
| 2 |
Alleman, Landon H (10)
|
ILUPDT |
2:25.46 |
2:23.03 |
-2.43 |
| 3 |
Zhong, Austin Z (10)
|
OHRAYS |
2:26.86 |
2:30.00 |
3.14 |
| 4 |
Knoblauch, Tate E (10)
|
INSCA |
2:33.32 |
2:31.11 |
-2.21 |
| 5 |
Broxterman, Samuel D (10)
|
OHRAYS |
2:39.28 |
2:35.85 |
-3.43 |
| 6 |
Francis, Eli R (10)
|
INFAST |
2:38.56 |
2:40.00 |
1.44 |
| 7 |
Xu, Jake D (9)
|
OHRAYS |
2:40.64 |
2:41.03 |
0.39 |
| 8 |
Conner, Blake C (10)
|
INNOB |
2:41.71 |
2:42.28 |
0.57 |
| 9 |
Vohs, Isaiah P (10)
|
INNOB |
2:46.59 |
2:42.76 |
-3.83 |
| 10 |
Wang, Alexander (10)
|
OHRAYS |
3:22.72 |
2:49.04 |
-33.68 |
| 11 |
Monger, Brady R (9)
|
INNOB |
2:52.50 |
2:50.27 |
-2.23 |
| 12 |
Collins, Henry J (10)
|
INSCA |
2:56.57 |
2:55.58 |
-0.99 |
| 13 |
McCoy, Nicholas J (10)
|
INGRE |
3:08.90 |
2:56.54 |
-12.36 |
| 14 |
Gwinn, Noah W (9)
|
ILHEAT |
|
2:57.52 |
- |
| 15 |
Smith, Liam J (9)
|
INSCA |
|
2:57.64 |
- |
| 16 |
Upshaw, Qwinton I (10)
|
INFAST |
3:10.72 |
3:06.25 |
-4.47 |
| 17 |
Pathak, Tavish (10)
|
OHRAYS |
2:59.74 |
3:07.87 |
8.13 |
| 18 |
McClintock, Tonatiuh A (10)
|
INWAC |
3:25.60 |
3:11.18 |
-14.42 |
| 19 |
Teske, Jackson J (9)
|
INNOB |
3:09.50 |
3:12.77 |
3.27 |
| 20 |
Van Sant, Dillon M (9)
|
INBEST |
3:36.35 |
3:15.68 |
-20.67 |
| 21 |
Makielski, Matthew C (9)
|
INFAST |
3:23.18 |
3:18.32 |
-4.86 |
| 22 |
Stagg, Eli (9)
|
OHLIFE |
|
3:22.73 |
- |
| 23 |
Gannon, Gavin M (10)
|
INFAST |
3:21.10 |
3:24.95 |
3.85 |
| 24 |
Shreve, Owen (10)
|
INJAGS |
3:51.49 |
3:31.18 |
-20.31 |
| 25 |
Chandler, Tristen Q (10)
|
INYSC |
4:17.89 |
3:33.59 |
-44.30 |
| 26 |
Lee, Nathan A (9)
|
OHRAYS |
|
3:39.30 |
- |