| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tacik, Morgan E (19)
|
NCTYDE |
39.03 |
39.16 |
0.13 |
| 2 |
Bunting, Meredith T (15)
|
NCTYDE |
39.87 |
39.41 |
-0.46 |
| 3 |
Jones, Paige L (16)
|
NCTYDE |
41.68 |
40.18 |
-1.50 |
| 4 |
Chang, Amy (16)
|
NCRSA |
42.03 |
40.92 |
-1.11 |
| 5 |
Marston, Rachel C (18)
|
NCRSA |
40.37 |
40.98 |
0.61 |
| 6 |
Wolf, Anna K (15)
|
NCTYDE |
43.65 |
41.37 |
-2.28 |
| 7 |
Hooten, Bailey H (17)
|
NCTYDE |
38.37 |
41.54 |
3.17 |
| 8 |
Joram, Sarah M (17)
|
NCTYDE |
39.19 |
41.66 |
2.47 |
| 9 |
Conroy, Erin P (18)
|
NCRSA |
43.14 |
41.91 |
-1.23 |
| 10 |
McGill, Meredith D (17)
|
NCHPSC |
42.72 |
42.22 |
-0.50 |
| 11 |
Link, Madison G (15)
|
NCRSA |
|
42.23 |
- |
| 12 |
Hall, Kristina L (15)
|
NCTYDE |
45.99 |
42.97 |
-3.02 |
| 13 |
Blackwell, Madeline C (15)
|
NCHPSC |
1:04.52 |
46.39 |
-18.13 |
| 14 |
Ercolino, Gabriella T (15)
|
NCRSA |
1:05.78 |
47.88 |
-17.90 |
|
Bowman, Elizabeth (17)
|
NCHPSC |
38.00 |
NS |
- |