| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bruno, Luke A (11)
|
GUPFL |
33.67 |
33.37 |
-0.30 |
| 2 |
Brenes-Quesada, Javier (12)
|
GUPFL |
40.24 |
35.81 |
-4.43 |
| 3 |
Ong, Ian S (12)
|
GUDADS |
38.10 |
36.45 |
-1.65 |
| 4 |
Vasquez, Gabriel L (11)
|
GUDADS |
36.92 |
37.28 |
0.36 |
| 5 |
Vogel, Jackson W (11)
|
GUESCA |
39.52 |
38.85 |
-0.67 |
| 6 |
Saxe, Micah D (11)
|
GUDADS |
40.08 |
39.82 |
-0.26 |
| 7 |
Tran, Matthew V (12)
|
GUTASC |
43.09 |
40.58 |
-2.51 |
| 8 |
Bustos, Santiago C (12)
|
GUDADS |
40.26 |
41.13 |
0.87 |
| 9 |
Puecher, Alan (12)
|
GUPFL |
42.65 |
41.20 |
-1.45 |
| 10 |
Liu, David (11)
|
GUPFL |
41.57 |
41.44 |
-0.13 |
| 11 |
Cogliatti, Gabriel K (12)
|
GULIFE |
43.59 |
42.32 |
-1.27 |
| 12 |
Nadkarni, Aarin (12)
|
GUPFL |
44.78 |
42.79 |
-1.99 |
| 13 |
Lin, Asher (11)
|
GUPFL |
41.09 |
43.90 |
2.81 |
| 14 |
Vo, James * (11)
|
GUTASC |
40.89 |
44.35 |
3.46 |
| 15 |
Maloney, Cian F (11)
|
GUPFL |
48.37 |
45.13 |
-3.24 |
| 16 |
Gulley, Zachary J (12)
|
GUBATS |
46.24 |
45.99 |
-0.25 |
| 17 |
Rodriguez, Jacob A (11)
|
GUBATS |
59.71 |
54.47 |
-5.24 |
|
Tran, Dylan M (11)
|
GUTASC |
45.45 |
DQ |
- |
|
Bradshaw, Zachary L (11)
|
GUPFL |
57.45 |
NS |
- |
|
Zhang, Ethan A (11)
|
GUDADS |
|
NS |
- |
|
Marino, Noah K (12)
|
GUDADS |
45.84 |
NS |
- |
|
Bowen, Oliver C (12)
|
GUSPA |
1:27.83 |
NS |
- |
|
Gonzalez, Adrian J (12)
|
GUDADS |
41.03 |
NS |
- |
|
Bowlin, Everett N (11)
|
GUDADS |
|
NS |
- |