| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Osio, Riccardo S (12)
|
NTLAC |
31.68 |
28.40 |
-3.28 |
| 2 |
Kincheloe, Carter R (12)
|
NTLAC |
30.95 |
30.18 |
-0.77 |
| 3 |
Goins, Solomon T (12)
|
NTMAC |
31.26 |
30.94 |
-0.32 |
| 4 |
Stanislaus, Maxwell S (12)
|
NTLAC |
30.74 |
31.23 |
0.49 |
| 5 |
Schwendinger, Jacob T (12)
|
NTLAC |
32.19 |
31.79 |
-0.40 |
| 6 |
Temiz, Able C (12)
|
NTLAC |
32.27 |
31.88 |
-0.39 |
| 7 |
Peng, Jackson B (12)
|
NTLAC |
35.06 |
32.53 |
-2.53 |
| 8 |
Roybal, Hudson L (11)
|
NTLAC |
32.98 |
33.24 |
0.26 |
| 9 |
Cormack, Eason C (12)
|
NTNTN |
35.01 |
33.27 |
-1.74 |
| 10 |
Boeck, Andrew M (12)
|
NTNTN |
34.28 |
33.93 |
-0.35 |
| 11 |
LaClair, Jack L (12)
|
NTLAC |
33.06 |
34.10 |
1.04 |
| 12 |
Batuna, Jason C (12)
|
NTLAC |
33.85 |
34.31 |
0.46 |
| 13 |
Klubben, William B (11)
|
NTLAC |
34.06 |
34.68 |
0.62 |
| 14 |
Murthy, Dhanush (11)
|
NTLAC |
39.52 |
35.08 |
-4.44 |
| 15 |
Currey, William P (12)
|
NTLAC |
34.91 |
35.18 |
0.27 |
| 16 |
Chiang, Tyler B (11)
|
NTLAC |
34.58 |
36.25 |
1.67 |
|
Wester, Christian M (11)
|
NTLAC |
40.49 |
DQ |
- |
|
Kim, Danny (11)
|
NTLAC |
39.65 |
NS |
- |
|
Jackson, Grant J (12)
|
NTLAC |
35.19 |
NS |
- |