| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clouser, Kristiana I (14)
|
NITTSC |
3:09.18 |
3:03.45 |
-5.73 |
| 2 |
Hesch, Natalie G (14)
|
NITTSC |
3:16.21 |
3:07.24 |
-8.97 |
| 3 |
Lee, Madeline E (14)
|
NIVELO |
2:57.30 |
3:08.18 |
10.88 |
| 4 |
Fritsch, Natalie A (14)
|
NITTSC |
3:12.37 |
3:14.87 |
2.50 |
| 5 |
Chaplin, Allison V (14)
|
NITTSC |
3:18.28 |
3:16.76 |
-1.52 |
| 6 |
Roberts, Alaina G (14)
|
NITTSC |
3:27.88 |
3:30.16 |
2.28 |
| 7 |
Lin, Isabella E (13)
|
NIVELO |
3:07.06 |
3:31.47 |
24.41 |
| 8 |
Del Regno, Jaina R (13)
|
NIRAYS |
|
3:41.70 |
- |
| 9 |
Canestaro, Annabelle S (14)
|
NIVELO |
3:23.93 |
3:43.90 |
19.97 |
| 10 |
Chelpinski, Angelica J (13)
|
NIVELO |
3:37.81 |
3:49.84 |
12.03 |
| 11 |
Truby, Jenna M (13)
|
NIHHSC |
|
3:58.60 |
- |
| 12 |
Nadarajan, Gouripriya (13)
|
NIVELO |
4:00.64 |
3:59.47 |
-1.17 |