| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Mira (12)
|
NIVELO |
29.39 |
33.64 |
4.25 |
| 2 |
Barros, Rachel (12)
|
NIUN-B |
31.99 |
35.17 |
3.18 |
| 3 |
Scibetta, Madeline C (12)
|
NITTSC |
43.58 |
39.12 |
-4.46 |
| 4 |
Lyons, Lucy E (11)
|
NITTSC |
40.48 |
39.40 |
-1.08 |
| 5 |
Shepherd, Taylor R (11)
|
NITTSC |
42.28 |
40.77 |
-1.51 |
| 6 |
Bronson, Madeline J (12)
|
NITTSC |
44.08 |
40.99 |
-3.09 |
| 7 |
Roeser, Lila J (12)
|
NITTSC |
45.14 |
41.13 |
-4.01 |
| 8 |
Gentile, Olivia (12)
|
NIVELO |
37.47 |
43.15 |
5.68 |
| 9 |
Morrissey, Erin E (11)
|
NITTSC |
44.36 |
44.59 |
0.23 |
| 10 |
Winker, Delilah M (11)
|
NITTSC |
47.90 |
46.22 |
-1.68 |
| 11 |
Fabiano, Mackenzie G (11)
|
NIHHSC |
46.02 |
46.64 |
0.62 |
| 12 |
Sperry, Lola E (12)
|
NITTSC |
50.50 |
49.50 |
-1.00 |
| 13 |
Hesch, Addison L (11)
|
NITTSC |
47.09 |
50.48 |
3.39 |
|
McCann, Madaline S (11)
|
NIRAYS |
|
NS |
- |
|
Hennessey, Caitlin G (11)
|
NIVELO |
|
NS |
- |