| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mitcham, Avery J (11)
|
NCGCY |
31.94 |
31.03 |
-0.91 |
| 2 |
Ross, Ian B (12)
|
NCTYDE |
31.77 |
31.11 |
-0.66 |
| 3 |
Crowley, George L (11)
|
NCTYDE |
33.61 |
32.24 |
-1.37 |
| 4 |
Lee, Jaidon J (12)
|
NCTYDE |
32.63 |
32.29 |
-0.34 |
| 5 |
Anderson, Iv, Benjamin F (12)
|
NCRACY |
33.34 |
35.18 |
1.84 |
| 5 |
Wang, Wanbo (12)
|
NCTYDE |
35.38 |
35.18 |
-0.20 |
| 7 |
Doss, Daniel M (11)
|
NCYBAC |
34.23 |
35.79 |
1.56 |
| 8 |
Kafitz, Brandon J (11)
|
NCRACY |
31.07 |
36.11 |
5.04 |
| 9 |
Thompson, Kaden J (11)
|
NCRACY |
40.80 |
36.15 |
-4.65 |
| 10 |
Kohrt, Jason P (12)
|
NCTYDE |
35.21 |
36.85 |
1.64 |
| 11 |
McBride, Joshua (11)
|
NCRACY |
|
46.99 |
- |
|
Hawkins III, Rodney D (12)
|
NCRACY |
33.96 |
NS |
- |